https://ideawaza.com/api.php?action=feedcontributions&feedformat=atom&user=128.32.0.0%2F16IdeaWazaWiki - User contributions [en]2026-09-29T21:38:03ZUser contributionsMediaWiki 1.46.0https://ideawaza.com/index.php?title=Phosphate_buffered_saline&diff=41801Phosphate buffered saline2006-06-06T19:56:09Z<p>128.32.181.136: Made minor grammar/syntax changes</p>
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<div>'''Phosphate buffered saline''' (abbreviated PBS) is a [[buffer solution]] commonly used in [[biochemistry]]. It is a salty solution containing sodium chloride, sodium phosphate and potassium phosphate. The buffer helps to maintain a constant pH. The concentration usually matches the human body (isotonic).<br />
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PBS has many uses because it is isotonic and non-toxic to cells. It can be used to dilute substances.<br />
It is used as a cellular cleaning solution. In addition, PBS is also used to disengage attached and clumped cells.<br />
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To make a 10 liter stock of 10x PBS:<br />
#weigh the following<br />
::*800g NaCL<br />
::*20g KCL<br />
::*144g Na2HPO4<br />
::*24g KH2HPO4<br />
::*24g KH2PO4<br />
#add and dissolve in 8L of distilled water under mixing<br />
#adjust PH to 7.4 with concentrated HCl under constant mixing<br />
#fill up to 10 liters with distilled water<br />
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{{biochem-stub}}<br />
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[[Category:Buffers]]</div>128.32.181.136