https://ideawaza.com/api.php?action=feedcontributions&feedformat=atom&user=66.91.98.16IdeaWazaWiki - User contributions [en]2026-09-29T19:33:19ZUser contributionsMediaWiki 1.46.0https://ideawaza.com/index.php?title=Phosphate_buffered_saline&diff=41810Phosphate buffered saline2006-09-18T03:38:25Z<p>66.91.98.16: </p>
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<div>'''Phosphate buffered saline''' (abbreviated PBS) is a [[buffer solution]] commonly used in [[biochemistry]]. It is a salty solution containing sodium chloride, sodium phosphate and potassium phosphate. The buffer helps to maintain a constant pH. The concentration usually matches the human body (isotonic).<br />
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PBS has many uses because it is isotonic and non-toxic to cells. It can be used to dilute substances.<br />
It is used as a cellular cleaning solution. <br />
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Additives can be used to add function. For example, PBS with [[EDTA]] is also used to disengage attached and clumped cells. Divalent [[metals]] such as [[zinc]], however, cannot be added as this will cause precipitation. For these sorts of applications, [[good buffers]] are recommended.<br />
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To make a 10 liter stock of 10x PBS:<br />
#weigh the following<br />
::*800 g NaCl<br />
::*20 g KCl<br />
::*144 g Na<sub>2</sub>HPO<sub>4</sub><br />
::*24 g KH<sub>2</sub>PO<sub>4</sub><br />
#add and dissolve in 8 L of distilled water under mixing<br />
#pH is ~6.8, but when you dilute the 10x PBS to 1x PBS it should change to 7.4 (try with a little sample; Phosphate buffers change their pH with the dilution) <br />
#fill up to 10 L with distilled water<br />
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{{biochem-stub}}<br />
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[[Category:Buffers]]</div>66.91.98.16