Eukaryotic transcription: Difference between revisions
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The Nobel Prize in Chemistry in 2006 was awarded to [[w:Roger D. Kornberg|Roger D. Kornberg]] for his work on the molecular basis of '''eukaryotic transcription''' <ref name=Nobel2006">2006 award at the [http://nobelprize.org/nobel_prizes/chemistry/laureates/2006/index.html Nobel Prize website].</ref>. | The Nobel Prize in Chemistry in 2006 was awarded to [[w:Roger D. Kornberg|Roger D. Kornberg]] for his work on the molecular basis of '''eukaryotic transcription''' <ref name=Nobel2006">2006 award at the [http://nobelprize.org/nobel_prizes/chemistry/laureates/2006/index.html Nobel Prize website].</ref>. | ||
==Transcription== | |||
Roger Kornberg's laboratory has used biochemical techniques to isolate the large complex of proteins that allow RNA polymerase II to produce messenger RNA. Working with [[w:Yeast|yeast]] cells as an experimental system, Kornberg's research team has isolated and purified the functioning RNA pol II protein complex with attached template | |||
[[w:DNA|DNA]], product [[w:mRNA|mRNA]] and substrate [[w:Nucleotide|nucleotides]] and captured images of the complex using [[w:Electron microscope|electron microscopy]] and [[w:X-ray crystallography|X-ray crystallography]] <ref name="Thelander2006">Advanced information on the Nobel Prize in Chemistry 2006: [http://www.kva.se/KVA_Root/files/newspics/DOC_200610494343_28494252634_chemadv06.pdf Molecular basis of eukaryotic transcription] by Lars Thelander.</ref> | |||
==References== | ==References== | ||
Revision as of 15:07, 4 October 2006
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The Nobel Prize in Chemistry in 2006 was awarded to Roger D. Kornberg for his work on the molecular basis of eukaryotic transcription [2].
Transcription
Roger Kornberg's laboratory has used biochemical techniques to isolate the large complex of proteins that allow RNA polymerase II to produce messenger RNA. Working with yeast cells as an experimental system, Kornberg's research team has isolated and purified the functioning RNA pol II protein complex with attached template DNA, product mRNA and substrate nucleotides and captured images of the complex using electron microscopy and X-ray crystallography [3]
References
- ↑ Structural basis of transcription: an RNA polymerase II elongation complex at 3.3 A resolution by Averell L. Gnatt, Patrick Cramer, Jianhua Fu, David A. Bushnell and Roger D. Kornberg in Science (2001) Volume 292, pages 1876-1882.
- ↑ 2006 award at the Nobel Prize website.
- ↑ Advanced information on the Nobel Prize in Chemistry 2006: Molecular basis of eukaryotic transcription by Lars Thelander.
See also
- This lesson on RNA interference is part of the Nobel Prize in Chemistry learning project.
- The Nobel Prize in Physiology or Medicine learning project.
External links
- RNA polymerase II article at Wikipedia.