Eukaryotic transcription

Figure 2. Eukaryotic RNA polymerase II in a complex with DNA and mRNA .

The Nobel Prize in Chemistry in 2006 was awarded to Roger D. Kornberg for his work on the molecular basis of eukaryotic transcription .

Transcription

File:Information flow.png

Figure 1. Information flow in living cells from DNA to RNA to protein.

Eukaryotic organisms such as humans store their genetic information in the structure of DNA molecules. Most of the genetic instructions in genes are used to specify the structures of proteins (Figure 1). The many proteins of cells function as molecular machines that produce the living state. Intermediate between DNA and protein is RNA. The process by which cells convert the structure of a gene into the structure of a mRNA molecule is called transcription. In eukaryotes, a large molecular complex of specialized proteins is required to achieve the careful control of which subset of genes is transcribed in each type of cell. At the core of this complex is the enzyme RNA polymerase II.

Roger Kornberg's laboratory has used biochemical techniques to isolate the large complex of proteins that allow RNA polymerase II to produce messenger RNA. Working with yeast cells as an experimental system, Kornberg's research team has isolated and purified the functioning RNA pol II protein complex with attached template DNA, product mRNA and substrate nucleotides and captured images of the complex using electron microscopy and X-ray crystallography (see Figure 2)

Figure 3. A phylogenetic tree of living things, based on rRNA sequence data, showing the separation of bacteria, archaea, and eukaryotes .

General transcription factors

There are three major forms of life on Earth, bacteria, archaea and eukaryotes (Figure 3). RNA polymerase in bacteria is less complex than RNA polymerase in eukaryotes. Some of the increased complexity of RNA polymerase in eukaryotes reflects differences between DNA in eukaryotes and DNA in bacteria. Two important differences are that eukaryotes organize their DNA into nucleosomes and have more complex mechanisms for regulation of gene transcription.

File:Nucleosome rasmol.JPG

Figure 4. The DNA of eukaryotes wraps around histone proteins forming nucleosomes. .

Nucleosomes are a complex of DNA and histone proteins (Figure 4). In order for transcription to occur, DNA must be released from being tightly coiled in nucleosomes. Bacteria do not have nucleosomes. Another complication of eukaryotic gene expression regulation is that gene sequences controlling transcription are often distant from the DNA site where transcription starts. The RNA polymerase of bacteria is relatively small with a core of five protein subunits and one additional protein that recognizes the start points for transcription. In contrast, RNA polymerase II of yeast (Baker’s yeast Saccharomyces cerevisiae) has 12 protein subunits and requires five general transcription factor proteins (TFIIB, D, E, F and H). The general transcription factors are complex, for example, TFIIH has at least six protein subunits in various eukaryotic organisms from yeast to mammals. In addition, another large protein complex called Mediator is involved in the control of RNA polymerase II . In some some cells, Mediator is about 4 times larger than the RNA polymerase core complex (with as many as 20 different protein subunits) and is important for transmitting the effects of positive and negative regulators of gene transcription (often quite distant from the transcription start site) to the core polymerase.

See also

• This lesson on RNA interference is part of the Nobel Prize in Chemistry learning project.
• The Nobel Prize in Physiology or Medicine learning project.

External links

• RNA polymerase II article at Wikipedia.