Phosphate buffered saline: Difference between revisions
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PBS has many uses because it is isotonic and non-toxic to cells. It can be used to dilute substances. | PBS has many uses because it is isotonic and non-toxic to cells. It can be used to dilute substances. | ||
It is used as a cellular cleaning solution. In addition, PBS is also used to disengage attached and clumped cells. | It is used as a cellular cleaning solution. In addition, PBS is also used to disengage attached and clumped cells. | ||
To make a 10 liter stock of 10 times PBS use | |||
#weigh the following | |||
*800g NaCL | |||
*20g KCL | |||
*144g Na2HPO4 | |||
*24g KH2HPO4 | |||
*24g KH2PO4 | |||
#add and dissolve to 8 l of distilled water under mixing | |||
#adjust PH to 7,4 with concentrated HCL under constant mixing | |||
#fill up to 10 liters with distilled water | |||
{{biochem-stub}} | {{biochem-stub}} | ||
[[Category:Buffers]] | [[Category:Buffers]] | ||
Revision as of 12:08, 31 May 2006
Phosphate buffered saline (abbreviated PBS) is a buffer solution commonly used in biochemistry. It is a salty solution containing sodium chloride, sodium phosphate and potassium phosphate. The buffer helps to maintain a constant pH. The concentration usually matches the human body (isotonic).
PBS has many uses because it is isotonic and non-toxic to cells. It can be used to dilute substances. It is used as a cellular cleaning solution. In addition, PBS is also used to disengage attached and clumped cells.
To make a 10 liter stock of 10 times PBS use
- weigh the following
- 800g NaCL
- 20g KCL
- 144g Na2HPO4
- 24g KH2HPO4
- 24g KH2PO4
- add and dissolve to 8 l of distilled water under mixing
- adjust PH to 7,4 with concentrated HCL under constant mixing
- fill up to 10 liters with distilled water