Phosphate buffered saline: Difference between revisions
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To make a 10 liter stock of 10 times PBS use | To make a 10 liter stock of 10 times PBS use | ||
#weigh the following | #weigh the following | ||
*800g NaCL | ::*800g NaCL | ||
*20g KCL | ::*20g KCL | ||
*144g Na2HPO4 | ::*144g Na2HPO4 | ||
*24g KH2HPO4 | ::*24g KH2HPO4 | ||
*24g KH2PO4 | ::*24g KH2PO4 | ||
#add and dissolve to 8 l of distilled water under mixing | #add and dissolve to 8 l of distilled water under mixing | ||
#adjust PH to 7,4 with concentrated HCL under constant mixing | #adjust PH to 7,4 with concentrated HCL under constant mixing | ||
Revision as of 12:08, 31 May 2006
Phosphate buffered saline (abbreviated PBS) is a buffer solution commonly used in biochemistry. It is a salty solution containing sodium chloride, sodium phosphate and potassium phosphate. The buffer helps to maintain a constant pH. The concentration usually matches the human body (isotonic).
PBS has many uses because it is isotonic and non-toxic to cells. It can be used to dilute substances. It is used as a cellular cleaning solution. In addition, PBS is also used to disengage attached and clumped cells.
To make a 10 liter stock of 10 times PBS use
- weigh the following
- 800g NaCL
- 20g KCL
- 144g Na2HPO4
- 24g KH2HPO4
- 24g KH2PO4
- add and dissolve to 8 l of distilled water under mixing
- adjust PH to 7,4 with concentrated HCL under constant mixing
- fill up to 10 liters with distilled water