Phosphate buffered saline: Difference between revisions
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::*24 g KH<sub>2</sub>PO<sub>4</sub> | ::*24 g KH<sub>2</sub>PO<sub>4</sub> | ||
#add and dissolve in 8 L of distilled water under mixing | #add and dissolve in 8 L of distilled water under mixing | ||
# | #pH is ~6.8, but when you dilute the 10x PBS to 1x PBS it should change to 7.4 (try with a little sample; Phosphate buffers change their pH with the dilution) | ||
#fill up to 10 L with distilled water | #fill up to 10 L with distilled water | ||
{{biochem-stub}} | {{biochem-stub}} | ||
[[Category:Buffers]] | [[Category:Buffers]] | ||
Revision as of 14:56, 19 July 2006
Phosphate buffered saline (abbreviated PBS) is a buffer solution commonly used in biochemistry. It is a salty solution containing sodium chloride, sodium phosphate and potassium phosphate. The buffer helps to maintain a constant pH. The concentration usually matches the human body (isotonic).
PBS has many uses because it is isotonic and non-toxic to cells. It can be used to dilute substances. It is used as a cellular cleaning solution. In addition, PBS is also used to disengage attached and clumped cells.
To make a 10 liter stock of 10x PBS:
- weigh the following
- 800 g NaCl
- 20 g KCl
- 144 g Na2HPO4
- 24 g KH2PO4
- add and dissolve in 8 L of distilled water under mixing
- pH is ~6.8, but when you dilute the 10x PBS to 1x PBS it should change to 7.4 (try with a little sample; Phosphate buffers change their pH with the dilution)
- fill up to 10 L with distilled water