Phosphate buffered saline
Phosphate buffered saline (abbreviated PBS) is a buffer solution commonly used in biochemistry. It is a salty solution containing sodium chloride, sodium phosphate and potassium phosphate. The buffer helps to maintain a constant pH. The concentration usually matches the human body (isotonic).
PBS has many uses because it is isotonic and non-toxic to cells. It can be used to dilute substances. It is used as a cellular cleaning solution. In addition, PBS is also used to disengage attached and clumped cells.
To make a 10 liter stock of 10x PBS:
- weigh the following
- 800g NaCL
- 20g KCL
- 144g Na2HPO4
- 24g KH2HPO4
- 24g KH2PO4
- add and dissolve in 8L of distilled water under mixing
- adjust PH to 7.4 with concentrated HCl under constant mixing
- fill up to 10 liters with distilled water